Figure 2.

Photomicrographs showing apoptosis of osteocytes as a marker of early osteonecrosis of the femoral head. TUNEL staining apoptosis assay analyzed by (A): florescence microscope, (B): superimposed florescence and optical view on femoral head in II: Lewis, III: Fisher, IV: Wistar Kyoto, V: Wistar Furth and VI: Spontaneous Hypertensive rats induced with steroids for 3 months versus a control sample (I). The nucleus of apoptotic cells are shown in green. Magnification ×200.