Figure 3.

Histological evaluation of in vitro constructs. Fibrin/PLGA constructs showed superior histological features of cartilage-like tissue compared to PLGA. Differences between fibrin/PLGA (Figure 3A, B, C and Figure 3G, H, I) and PLGA (Figure 3D, E, F and Figure 3J, K, L) were clearly visible in term of overall cartilaginous tissue formation, cells organization and ECM distribution. The fibrin/PLGA constructs was intensely stained with Safranin O for accumulated proteoglycan and Alcian Blue for GAG at 2 weeks and greatest at 3 weeks.