Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells

doi:10.1186/1749-799X-2-27

Journal of Orthopaedic Surgery and Research

Volume 2

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Wildemann B
Burkhardt N
Luebberstedt M
Vordemvenne T
Schmidmaier G

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Burkhardt N
Luebberstedt M
Vordemvenne T
Schmidmaier G
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Research article

Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells

Britt Wildemann¹^,2 , Nicole Burkhardt¹ , Marc Luebberstedt¹ , Thomas Vordemvenne³ and Gerhard Schmidmaier¹^,2

¹Center for Musculoskeletal Surgery, Charité-Universitätsmedizin Berlin, Germany

²Berlin-Brandenburg Center for Regenerative Therapies, Berlin, Germany

³Dept. Trauma, Hand and Reconstructive Surgery, University Hospital, Muenster, Germany

author email corresponding author email

Journal of Orthopaedic Surgery and Research 2007, 2:27doi:10.1186/1749-799X-2-27


Published:	20 December 2007

Abstract

Growth factors are in clinical use to stimulate bone growth and regeneration. BMP-2 is used in long bone and spinal surgery, PDGFbb for the treatment of periodontal defects and children with growth hormone receptor deficiency are treated with IGF-I.

Aim of the present study was the comparative analysis of the effect of these growth factors released from a local drug delivery system on cells of the osteogenic lineage at differing differentiation stages.

The experiments with the mesenchymal cell line C2C12 revealed a proliferating effect of all three growth factors and a differentiating effect of BMP-2 with a dramatic increase in alkaline phosphatase activity. None of the growth factors stimulated cell migration.

Human osteoblast like cells showed similar results with an increase in proliferation after stimulation with IGF-I or PDGFbb. The enzymatic activity of alkaline phosphatase was enhanced only in the cells stimulated with BMP-2. This group showed also more mineralized matrix compared to the other groups.

In conclusion, the growth factors IGF-I and PDGFbb delivered with a local drug delivery system stimulated cell proliferation, whereas BMP-2 showed a dramatic effect on differentiation on osteoblast precursor cells and osteoblast like cells.